primary antibodies against nr1  (GeneTex)

Journal: Frontiers in Neuroscience

Article Title: Repetitive Pain in Neonatal Male Rats Impairs Hippocampus-Dependent Fear Memory Later in Life

doi: 10.3389/fnins.2020.00722

Figure Lengend Snippet: Primer sequences used for mRNA quantification by real-time PCR.

Article Snippet: Then the membranes were blocked in 5% non-fat milk for 1.5 h at room temperature, followed by incubation overnight at 4°C with a primary antibody against NR1 (1:500, Santa Cruz), NR2A (1:500, Santa Cruz), NR2B (1:500, Santa Cruz), GluR1 (1:1000, Millipore), or β-actin (1:1000, Santa Cruz).

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Repetitive Pain in Neonatal Male Rats Impairs Hippocampus-Dependent Fear Memory Later in Life

doi: 10.3389/fnins.2020.00722

Figure Lengend Snippet: Hippocampal expression of synapse-related mRNAs and proteins. The independent t -test was used to detect group differences in mRNA and protein expression. The mRNA levels are presented as the ratio of each group to the control group, normalized to the corresponding GAPDH mRNA levels using the 2 – ΔΔCt method ( n = 10). The relative mRNA levels of NR2A (A) , NR2B (B) , GluR1 (C) and NR1 (D) were detected on P24 and P87. (E) Representative blots of hippocampal synapse-related proteins and Actin. (F) The protein expressions of NR2A, NR2B, GluR1 and NR1 were expressed as the fold change compared with the Actin ( n = 6). Data are expressed as the mean ± SEM. * P < 0.05, ** P < 0.01 for needle group vs. tactile group.

Article Snippet: Then the membranes were blocked in 5% non-fat milk for 1.5 h at room temperature, followed by incubation overnight at 4°C with a primary antibody against NR1 (1:500, Santa Cruz), NR2A (1:500, Santa Cruz), NR2B (1:500, Santa Cruz), GluR1 (1:1000, Millipore), or β-actin (1:1000, Santa Cruz).

Techniques: Expressing, Control

Journal: Scientific Reports

Article Title: Intermittent fasting uncovers and rescues cognitive phenotypes in PTEN neuronal haploinsufficient mice

doi: 10.1038/s41598-018-26814-6

Figure Lengend Snippet: Summary of western blotting statistical analyses.

Article Snippet: Briefly, samples (25 μg) were separated using 10% polyacrylamide gels, transferred onto nitrocellulose membranes, blocked with 5% BSA, and incubated with primary antibodies against the following targets: PTEN (54 kDa, 1:1000, #9559 Cell Signaling Technology, Danvers, MA, USA), AKT (55 kDa, 1:2000, #sc-1619 Santa Cruz Biotechnology, Dallas, TX, USA), p-AKT T308 (60 kDa, 1:750, #4056 Cell Signaling), p-AKT S473 (60 kDa, 1:750, #550747 BD Biosciences, San Jose, CA, USA), S6 (32 kDa, 1:1000, #2217 Cell Signaling), p-S6 (32 kDa, 1:1000, #5364 Cell Signaling), AMPA (100 kDa, 1:500, #13185 Cell Signaling), NR1 (116 kDa, 1:500, #G8913 Sigma-Aldrich, Saint Louis, MO, USA), NR2a (180 kDa, 1:1000, #4205 Cell Signaling), NR2b (190 kDa, 1:1000, #4207 Cell Signaling), PSD-95 (95 kDa, 1:750, #sc-71933 Santa Cruz), synaptophysin (38 kDa, 1:2000, #4329 Cell Signaling), and β-Actin (42 kDa, 1:10000, #A5441 Sigma-Aldrich).

Techniques: Western Blot